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Laatste wijziging: 24 January 2022
AGE mediated RAGE activity: new target for multiple sclerosis
Principal Investigator: Prof. Dr. Jerome Hendriks, Researcher: Suzanne Wetzels
The purpose of this study is to investigate the role of advanced glycation endproducts (AGE), the precursors of AGEs, methylglyoxal, and the receptor for AGEs (RAGE) in the pathophysiology of multiple sclerosis (MS). Advanced glycation endproducts (AGEs) are glycosylated proteins which originate from a non-enzymatic reaction of reduced sugars with amino acids to proteins through the formation of methylglyoxal. This reaction occurs in the body, but also in food that is heated. AGEs can bind to RAGE and trigger an inflammatory response.
In this study, plasma/serum, CSF samples and monocytes of MS patients and controls will be used. First, the concentration of AGEs and methylglyoxcal will be measured in the plasma/serum and CSF using ultra performance liquid chromatography tandem mass spectrometry (UPLC-MSMS). Next, the concentration of sRAGE will be determined in the plasma/serum and CSF using ELISA. Finally, peripheral blood mononuclear cells (PBMCs) will be used for FACS analysis and the amount of monocytes will be determined as will the degree of RAGE on these monocytes.