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Laatste wijziging: 12 januari 2022
Determination of metabolic profile of obese vs normal-weight children using 1H-NMR spectroscopy on blood and urine samples
Principal Investigator: Prof. Dr. Peter Adriaensens
In recent decades, the prevalence of overweight and obesity in children and adolescents has increased significantly. Childhood obesity increases the risk of developing insulin resistance (metabolic syndrome), type 2 diabetes, hepatic steatosis and / or premature heart failure. In addition, obese children are at increased risk to become obese adults. Early detection, prevention and intervention of obesity and associated health risks, is therefore very important.
The purpose of this cross-sectional study is to determine and compare the metabolic profile of 75 obese children with 75 normal-weight children, aged between 8 and 18 years of age, using 1 H-NMR spectroscopy on plasma and urine. Children eligible to participate in the study should not take any medication and should not have been seriously ill the last two weeks. They will be questioned (personal and family history, diet and physical activity), measured (height, weight, blood pressure, fitness, etc.) and 1 fasting urine and blood sample will be collected. The plasma and urine samples are analyzed using 1H-NMR spectrometry. 1H-NMR spectroscopic analysis will be combined with multivariate statistical analysis of the identified metabolites, in order to determine whether the metabolite composition of plasma and urine allows us to distinguished obese children from normal-weight children. The biochemical pathways in which the most distinctive metabolites are involved will be determined. The ultimate goal is to unravel early stage candidate biomarkers of obesity and correlated metabolic disorders, such as impaired glucose tolerance, insulin resistance and metabolic syndrome.
Bervoets L, Massa G. Classification and clinical characterization of metabolically "healthy" obese children and adolescents. J Pediatr Endocrinol Metab. 2016.
Bervoets L, Mewis A, Massa G. The shape of the plasma glucose curve during an oral glucose tolerance test as an indicator of Beta cell function and insulin sensitivity in end-pubertal obese girls.Horm Metab Res. 2015;47(6):445-51.
Bervoets L, Louis E, Reekmans G, Mesotten L, Thomeer M, Adriaensens P, Linsen L. Influence of preanalytical sampling conditions on the 1H NMR metabolic profile of human blood plasma and introduction of the Standard PREanalytical Code used in biobanking. Metabolomics. 2015; 11(5):1197-1207.
Bervoets L (PhD theses, Advisors: Massa G, Adriaensens P and Noben J. Study of the metabolic health of obese children and adolescents by clinical and metabolomics research. 2015.
Bervoets L, Massa G, Guedens W, Louis E, Noben JP, Adriaensens P. Metabolic profiling of type 1 diabetes mellitus in children and adolescents: a case-control study. Diabetol Metab Syndr. 2017;9:48.
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