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Laatste wijziging: 24 januari 2022
Study on the protein expression of collapsing response mediator protein 2 in the different T-cell populations
Principal Investigator: Prof. Dr. Niels Hellings, Researcher: Dr. Annelies Vanheel
In multiple sclerosis (MS), the migration of autoreactive T-cells to the central nervous system is one of the mechanisms that is responsible for the degradation of the myelin around the axons. Through this demyelination the signal transduction in the brain is disrupted, causing the typical symptoms of MS. Collapsin response mediator protein 2 (CRMP2) is a protein which may play a role in the migration of T-cells into the central nervous system (CNS).
The purpose of this study is to examine the expression of CRMP2 in the different T-cell populations that are involved in the disease process of MS, in order to gain a better understanding of the role of this protein in the migration of autoreactive T-cells to the CNS. Both the T-helper cells, cytotoxic T-cells, regulatory T-cells and memory T-cells will be studied. The CRMP2 expression will be compared between the different T-cell populations, as will the CRMP2 expression levels between healthy controls and patients with MS. A higher CRMP2 expression in a particular T-cell population, or in MS patients could indicate that an increased migration can take place.
In addition to the base CRMP2 expression of T-helper cells, we also want make a further division for the different subtypes of T-helper cells, namely T-helper 1, 2 and 17. Because these subtypes can not be measured in the blood, a differentiation experiment needs to be performed. To this end, T-helper cells will be stimulated by the addition of appropriate cytokines to proliferate to the different subtypes. The expression of CRMP2 will be measured via flow cytometric analysis, as well as by quantitative PCR.
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